SFN antibody
In stock
SKU/Catalog Number
PAab09897
Catalog Number: PAab09897
- Isotype IgG.
- Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.).
- Background/ FUNCTION.
- 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms, denoted 14-3-3 b, g, e, z, h, q and s, comprise this family of signaling intermediates. 14-3-3 s, also known as SFN, stratifin, HME1 or YWHAS, is a secreted adaptor protein that is involved in regulating both general and specific signaling pathways. Expressed predominately in stratified squamous keratinising epithelium, 14-3-3 s is able to bind and modify the activity of a large number of proteins, such as KRT17 (Keratin 17), through recognition of a phosphothreonine or phosphoserine motif. When bound to Keratin 17, for example, 14-3-3 s acts to stimulate the Akt/mTOR signaling pathway by upregulating protein synthesis and cell growth. 14-3-3 s also functions to positively mediate IGF-I-induced cell cycle progression and can bind to a variety of translation initiation factors, thus controlling mitotic translation. In response to tumor growth, 14-3-3 s positively regulates the tumor suppressor p53 and increases the rate of p53-regulated inhibition of G2/M cell cycle progression. Multiple isoforms of 14-3-3 s exist due to alternative splicing events.
- Immunogen 14-3-3 protein sigma.
- Specificity Human, Mouse, Rat.
- Tested Application ELISA, WB, IHC, IF.
- Recommended dilution WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:100.
- IHC use Immunohistochemistry of paraffin-embedded rat liver tissue slide using PAab09897( SFN antibody) at dilution of 1:100.
- WB use MCF-7 cells were subjected to SDS PAGE followed by western blot with PAab09897( SFN antibody) at dilution of 1:1000.
- Protein Information.
- Gene ID 2810.
- Uniprot ID P31947.
- Calculated MW 31 kDa.
- Research Area Neuroscience, Signal Transduction.
- Alternative Name 14 3 3 protein sigma, HME1, SFN, stratifin, YWHAS.
| Intended Use | Purification Immunogen affinity purified , Purity 95% as determined by SDS-PAGE. |
|---|---|
| Company | bioactiva diagnostica GmbH |
| Storage condition | 2-8°C |
| Product Number | PAab09897 |
| Manufacturer | Life Science Market |
| Certification | For research only |
| Order | Request Quote |
