HIF1a antibody
In stock
SKU/Catalog Number
PAab09940
Catalog Number: PAab09940
- Isotype IgG1
- Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)
- Background/ FUNCTION Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918).
- Immunogen hypoxia inducible factor 1, alpha subunit(basic helix-loop-helix transcription factor)
- Specificity Human
- Tested Application ELISA, IF, WB
- Recommended dilution WB: 1:1000-1:10000; IF: 1:50-1:200
- IHC use
- WB use
- Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with PAab09940(HIF1a Antibody) at dilution of 1:5000
- Protein Information
- Gene ID 3091
- Uniprot ID Q16665
- Calculated MW 120 kDa
- Research Area Signal Transduction, Metabolism, Cardiovascular, Developmental biology, Neuroscience, Stem Cells
- Alternative Name BHLHE78, MOP1, PASD8
| Intended Use | Purification protein A+G purified , Purity 95% as determined by SDS-PAGE |
|---|---|
| Company | bioactiva diagnostica GmbH |
| Storage condition | 2-8°C |
| Product Number | PAab09940 |
| Manufacturer | Life Science Market |
| Certification | For research only |
| Order | Request Quote |
