Immunoassays

This protocol details the procedure for testing samples with the OLM Aspergillus LFD (AspLFD). The following protocol is summarised in the figure overleaf. Please see the AspLFD handbook for more detailed product information.

The assay, based on the M5 and the M6 antibodies, measures total cell death due to apoptosis or necrosis and is intended for human serum or plasma.

The M30 CytoDeath™ ELISA offers a unique possibility to measure human cell death by apoptosis in cell cultures, multicellular spheroids and organ culture systems. The M30 CytoDeath™ ELISA is a product developed for cell culture applications, with a dynamic range and sensitivity suitable for in vitro work, making it a useful drug screening tool.

The M65 EpiDeath® ELISA represents the next generation of K18 positive biomarker assays.

The M65 EpiRat™ ELISA is based on the M5 and M6 antibodies, which bind to the biomarker Keratin-18 (K18). K18 is an intermediate filament protein in epithelial cells.