Plasmid classification: plant series, protein overexpression vector.
Plasmid size: 13832bp.
Plasmid label: N-10*His, N-EGFP, C-6*His.
Prokaryotic resistance: Kan.
Screening markers: Neo.
Cloned strain: DH5 alpha.
Culture conditions: 37 centigrade, aerobic LB.
5'sequencing primers: 35S:GACGCACAATCCCACTATCC.
3'sequencing primers: primers designed according to sequence.
Use: Plant expression.
pBI121-EGFP Description:
pBI121-EGFP is transformed from pBI121 and EGFP label. It is a plant expression vector with Kanamycin kanamycin resistance and can be transformed into Escherichia coli DH5 alpha. A large number of replication and amplification are screened by Kan. The culture condition is LB, 37. The promoter of the pBI121-EGFP carrier is 35S, which can be mediated by Agrobacterium tumefaciens to express EGFP green fluorescent protein in plant cells, and the label has N-10*His, N-EGFP, C-6*His, and can be purified with nickel column. The enzyme sites of pBI121-EGFP include XhoI, NcoI, SalI, SpeI, XbaI, SmaI, BamHI, SacI, EcoRI, which can be selected according to needs.