PBBR1MCS- 1 PLASMID

Name: PBBR1MCS- 1 PLASMID
Brand: bioactiva diagnostica GmbH
SKU: PVT5601

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SKU/Catalog Number

PVT5601

Katalognummer: PVT5601

pBBR1MCS-1 Information:
Promoter: Lac/lac, T3, T7, or clone promoter.
Replicator: pBBR1 Rep, pBBR1 oriV.
Plasmid classification: pBBR1MCS series plasmids.
Plasmid size: 4707bp.
Plasmid label: LacZ.
Prokaryotic resistance: chloramphenicol Chloramphenicol.
Cloned strain: DH5 alpha.
Culture conditions: 37 centigrade, aerobic, LB.
Host: a wide host.
Culture conditions: Reference Literature.
5'sequencing primers: M13R:CAGGAAACAGCTATGACC.
3'sequencing primers: M13F:TGTAAAACGACGGCCAGT.
Remarks: low copy plasmids.
A brief introduction of plasmids.
Use:pBBR1MCS Series plasmid.
pBBR1MCS-1 Description: Broad host shuttle plasmid pBBR1MCS-1 is constructed by Kovach, has confirmed that [2] can replicate in many Gram-negative bacteria, including Acetobacter xylinum, Alcaligenes eutrophus, Bartonella, bacilliformis (Bordetella spp.), pertussis (Brucella spp.), Caulobacter of Brucella crescentus, Escherichia coli, Pseudomonas fluorescens, Paracoccous denitrificans (Pseudomonas fluorescens) Pseudomonas putida (P., putida), alfalfa Rhizobium (Rhizobium meliloti), R. leguminosarum by. Viciae, rhodobactersphaeroides (Rhodobacter sphaeroides), Salmonella typhimurium (Salmonella, typhimurium) of Vibrio cholerae (Vibrio cholerae) and Xanthomonas campestris.
[1] Kovach ME, et al. pBBR1MCS: a broad-host-range cloning vector. Biotechniques, 1994,16 (5).
[2] Kovach ME, Elzer PH, Hill DS, et al. Four new, al., and PH.