PBAD/ GIII C

Name: PBAD/ GIII C
Brand: bioactiva diagnostica GmbH
SKU: PVT0715

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SKU/Catalog Number

PVT0715

Katalognummer: PVT0715

pBAD/gIII C Information:
Promoter: araBad.
Replicon: PBR322 ori.
Plasmid classification: Escherichia coli vector; pBAD series expression plasmid.
Plasmid size: 4149bp.
Plasmid label: N-GIII signal, C-His, C-Myc.
Prokaryotic resistance: ampicillin Amp.
Cloned strain: Top10.
Culture conditions: 37 centigrade, aerobic, LB.
Expressing the host: recommending LMG194.
Culture conditions: 37 centigrade, aerobic, LB.
Inducement: Arabia sugar.
5'sequencing primers: pBAD-F: ATGCCATAGCATTTTTATCC.
3'sequencing primers: pBAD-R: GATTTAATCTGTATCAGG.
pBAD/gIII C Description:
pBAD/gIII carrier is derived from pBR322 vector. The carrier is designed to regulate secretory expression and purify the recombinant protein in Escherichia coli. The recombinant protein was located using the gene III signal sequence to make it secreted into the periplasmic cavity of Escherichia coli. The use of Escherichia coli araBAD promoter (pBAD) enhanced the level of soluble expression of recombinant protein of Escherichia coli. The regulatory protein AraC on the carrier can regulate the pBad promoter.