p65/RELA antibody
Auf Lager
SKU/Catalog Number
PAab09875
Katalognummer: PAab09875
- Isotype IgG.
- Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.).
- Background/ FUNCTION.
- Nuclear factor k B(NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-Kb family in mammalian cells include the proto-oncogene c-Rel,p50/p105(NFkB1), p65(RelA), p52/p100(NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-Kb. The p65 subunit is a major component of NF-Kb complexes and is responsible for trans-activation. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. It associates with chromatin at the NF-kappa-B promoter region via association with DDX1.
- Immunogen v-rel reticuloendotheliosis viral oncogene homolog A(avian).
- Specificity Human, Rat.
- Tested Application ELISA, IHC, IF, WB.
- Recommended dilution WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200.
- IHC use Immunohistochemistry of paraffin-embedded rat lung using PAab09875(p65 antibody) at dilution of 1:50.
- WB use.
- HeLa cells were subjected to SDS PAGE followed by western blot with PAab09875(p65 antibody) at dilution of 1:500.
- Protein Information.
- Gene ID 5970.
- Uniprot ID Q04206.
- Calculated MW 65 kDa.
- Research Area Metabolism, Immunology, Cancer, Stem Cells, Cell Division and Proliferation.
- Alternative Name NFKB 3, NFKB p65, NFKB3, p65, RELA, Transcription factor p65.
| Intended Use | Purification Immunogen affinity purified , Purity 95% as determined by SDS-PAGE. |
|---|---|
| Company | bioactiva diagnostica GmbH |
| Storage condition | 2-8°C |
| Product Number | PAab09875 |
| Hersteller | Life Science Market |
| Zertifizierung | For research only |
| Order | Request Quote |
