lentiGuide-Puro

Name: lentiGuide-Puro
Brand: Bioactiva diagnostica GmbH
SKU: PVT6316

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SKU/Catalog Number

PVT6316

Katalognummer: PVT6316

lentiGuide-Puro Information.
Bacterial Resistance:Ampicillin.
Growth Strain.
Use:CRISPR-CAS25-sgRNA plasmid.
lentiGuide-Puro Description.
Vector backbone Custom.
Backbone size w/o insert (bp) 9482.
Vector type Mammalian Expression, Lentiviral, CRISPR.
Selectable markers Puromycin.
Gene/Insert name S. pyogenes sgRNA cassette.
Alt name S. pyogenes CRISPR customizable RNA element.
Species Synthetic.
Insert Size (bp) 100.
Promoter hU6.
Bacterial Resistance(s) Ampicillin.
Growth Temperature 37°C.
Growth Strain(s) Stbl3.
Growth instructions Use SapI digest to check for unwanted recombination of lentiviral plasmid. Only amplify in.
RecA- bacteria (eg. Stbl3).
Copy number High Copy.
Cloning method Restriction Enzyme.
5′ cloning site BsmBI (not destroyed).
3′ cloning site BsmBI (not destroyed).
5′ sequencing primer hU6-F.
3′ sequencing primer hGata4-rev (5'-ATTGTGGATGAATACTGCC-3').
Gene/Insert name Puromycin resistance.
Alt name puromycin N-acetyl-transferase.
Alt name PAC.
Insert Size (bp) 600.
Promoter Ef1-a.
Cloning method Restriction Enzyme.
5′ cloning site BsiWI (not destroyed).
3′ cloning site MluI (not destroyed).
5′ sequencing primer EF-1a-F.
3′ sequencing primer WPRE-R.