DNA2 antibody
Auf Lager
SKU/Catalog Number
PAab10066
Katalognummer: PAab10066
- Isotype IgG
- Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)
- Background/ FUNCTION
- Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex(RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break(DSB) repair: recruited by BLM and mediates the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA(ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function.
- Immunogen DNA replication helicase 2 homolog
- Specificity Human
- Tested Application ELISA, IHC
- Recommended dilution IHC: 1:20-1:200
- IHC use Immunohistochemistry of paraffin-embedded human colon cancer using PAab10066(DNA2 antibody) at dilution of 1:100
- WB use
- Protein Information
- Gene ID 1763
- Uniprot ID P51530
- Calculated MW 127 kDa
- Research Area Metabolism
- Alternative Name DNA2, DNA2L, KIAA0083
| Intended Use | Purification Immunogen affinity purified, Purity 95% as determined by SDS-PAGE |
|---|---|
| Company | bioactiva diagnostica GmbH |
| Storage condition | 2-8°C |
| Product Number | PAab10066 |
| Hersteller | Life Science Market |
| Zertifizierung | For research only |
| Order | Request Quote |
